Bacterial Pathogenesis

Spring 1999

(MCB 103, PLANT BI 103, PB HLTH 102)

MWF, 9-10 a.m., 102 Moffitt

Instructors

Daniel A. Portnoy, Course Director (10 weeks)

Office Hours: Tuesday: 10-12, 306 Barker Hall

Brian J. Staskawicz (5 weeks)

Enrollment

MCB undergraduates and graduates; Microbial Biology undergraduates; School of Public Health: MPH and Ph.D. students.

Course Control Numbers (CCNs): MCB: 58580/PLANT BI: 70905 /PB HLTH: 75527.

Course focus

This course for upper division and graduate students will explore the molecular and cellular basis of microbial pathogenesis. The course will focus on model microbial systems which illustrate mechanisms of pathogenesis. Most of the emphasis will be on bacterial pathogens of mammals and plants, but there may be some discussion of viral and protozoan pathogens. There will be an emphasis on experimental approaches. The course will also include some aspects of bacterial genetics and physiology, immune response to infection, and the cell biology of host-parasite interactions.

Grading

Three exams, each worth 1/3 of final grade

Text

• Bacterial Pathogenesis, A Molecular Approach by Abigail A. Salyers and Dixie D. Whitt, ASM Press.

Supplementary Texts

• Basic microbiology: Brock Biology of Microorganisms, 8th edition. Michael T. Madigan, John T. Martinko, and Jack Parker: Prentice-Hall.
• Basic immunology: Immunobiology: The Immune System in Health and Disease, Third edition.Charles A. Janeway, Jr. and Paul Travers, Current Biology Ltd. and Garland Publishing.
• Basic medical school microbiology and immunology: Microbiology, Fourth Edition. B. Davis, et al., Lippincott.

Microbial Biology Seminar Series

Students are encouraged to attend the Microbial Biology Seminar Series, Wednesdays from noon to 1 p.m. in 101 Barker Hall.

Course Schedule (last updated: 2/12/99)

January 20, Wed.	Overview of Bacterial Pathogenesis (Portnoy)
January 22, Fri.	The Bacterial Cell (Portnoy)
January 25, Mon.	Bacterial Growth (Portnoy)
January 27, Wed.	Antibiotics (Portnoy)
January 29, Fri.	Overview of Bacterial Gene Regulation (Staskawicz)
February 1, Mon.	Bacterial Molecular Genetics - Plasmids and Transposons (Portnoy)
February 3, Wed.	Humoral Immunity and Resistance to Complement (Portnoy)
February 5, Fri.	Cellular Immunity (Portnoy)
February 8, Mon.	Inflammation/Endotoxin (Portnoy)
February 10, Wed.	Experimental Approaches: Tools of the Trade (Portnoy)
February 12, Fri.	Genetic Approaches (Portnoy)
February 15, Mon.	President's Day: Holiday
February 17, Wed.	Avoidance of the Immune Response/Toxins and Pili (Portnoy)
February 19, Fri.	Diphtheria (Portnoy)
February 22, Mon.	Mid-Term Review
February 24, Wed.	MID-TERM EXAM
February 26, Fri.	Introduction to Plant-Bacteria Interactions (Staskawicz)
March 1, Mon.	Overview of Phytopathogenic Bacteria (Staskawicz)
March 3, Wed.	Diseases Caused by Phytopathogenic Bacteria (Staskawicz)
March 5, Fri.	Virulence Mechanisms of Phytopathogenic Bacteria; I. Toxins and Enzymes (Staskawicz)
March 8, Mon.	Virulence Mechanisms of Phytopathogenic Bacteria; II Secretion Systems (Staskawicz)
March 10, Wed.	Comparative Type III Secretion Systems in Phytopathogenic and Animal Bacterial Pathogens (Staskawicz)
March 12, Fri.	Pathogenicity Mechanisms of Phytopathogenic Bacteria; Introduction to the Hrp Locus (Staskawicz)
March 15, Mon.	Introduction to Agrobacterium Biology (Staskawicz)
March 17, Wed.	Molecular Mechanisms of T-DNA Delivery to Plant Cells (Staskawicz)
March 19, Fri.	Agrobacterium Binary Vector and Introduction to Rhizobium Biology (Staskawicz)
March 22-26	SPRING BREAK
March 29, Mon.	Nod Genes of Rhizobium and the Molecular Basis of Plant Nodulation (Staskawicz)
March 31, Wed.	Genetic Breeding for Plant Disease Resistance and Introduction to Molecular Genetics of Plant Disease Resistance (Staskawicz)
April 2, Fri.	Molecular Genetics and Signaling Events Involved in Plant Disease Resistance (Staskawicz)
April 5, Mon.	Mid-term review
April 7, Wed.	MID-TERM EXAM
April 9, Fri.	No Class
April 12, Mon.	Vibrio Cholerae (Portnoy)
April 14, Wed.	Vibrio Cholerae/ETEC (Portnoy)
April 16, Fri.	Pertussis (Portnoy)
April 19, Mon.	Bacterial Entry Mechanisms (Portnoy)
April 21, Wed.	Yersiniae /Invasion (Portnoy)
April 23, Fri.	Yersiniae/Yops (Portnoy)
April 26, Mon.	Listeria monocytogenes (Portnoy)
April 28, Wed.	Listeria monocytogenes (Portnoy)
April 30, Fri.	Shigellae (Portnoy)
May 3, Mon.	Salmonellae (Portnoy)
May 5, Wed.	Salmonellae (Portnoy)
May 7, Fri.	Legionella pneumophila (Portnoy)
May 10, Mon.	Tuberculosis (Portnoy)
May 12, Wed.	Gonorrhoea (Portnoy)
May 15, Saturday	FINAL: 8:00-11:00 a.m. in 101 Morgan